Browsing by Author "Mugampoza, D"

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Occurrence of listeria monocytogenes in bulked raw milk and traditionally fermented dairy products in Uganda
(African Journal of Food, Agriculture, Nutrition and Development., 2011) Mugampoza, D; Muyanja, CMBK; Ogwok, P; Serunjogi, ML; Nasinyama, GW
Bulked raw milk, locally processed yoghurt (LPY) and Bongo, a traditionally fermented dairy product sold at most informal milk cooling points in Uganda, were assessed for occurrence of Listeria spp. and Listeria monocytogenes. Total plate counts (TPC), holding temperature, pH and titratable acidity were also determined in all the milk products at the point of collection using standard methods. A total of 40 samples of bulked raw milk and 30 for each of LPY and Bongo were examined. Listeria spp. was higher in bulked raw milk than in fermented milk. Listeria spp. were detected in 60% of bulked raw milk, 30% of LPY and 15% of Bongo samples. Bulked raw milk had significantly higher (p<0.05) mean Listeria counts (3.10±0.06 log10 cfu mL-1) than LPY and Bongo, 0.82±0.18 and 0.32±0.18 log10 cfu mL-1, respectively. L. monocytogenes was isolated from 13 % of bulked raw milk, 3.0% of LPY but was not detectable in Bongo. Total plate count was significantly different (p<0.05) among the different milk types studied. Bongo had higher TPC (9.00±0.13 log10 cfu mL-1) than bulked raw milk (8.40±0.11 log10 cfu mL-1) and LPY (7.40±0.13 log10 cfu mL-1). The mean total plate counts (4.90 to 9.00±0.13 log10 cfu mL-1) of the fermented dairy products were within the acceptable limits for human consumption. The TPC for bulked raw milk (8.40±0.11 log10 cfu mL-1) was higher than the recommended values of national and international standards. Temperature, pH and titratable acidity were significantly different (p<0.05) among the different milk types. Holding temperature ranged from 5.40 to 8.60oC, pH was 4.20±0.04 to 6.10±0.04 whereas titratable acidity ranged from 0.22±0.01 to 089±0.01%. Listeria counts were not statistically predictable (p>0.05) from variation in the combined effect of pH, percent titratable acidity and temperature. Results of this study demonstrate a high risk associated with consumption of bulked raw milk and fermented dairy products in due to occurrence of Listeria spp.
Risk assessment for the occurrence of escherichia coli 0157:H7 in indigenous fermented milk (lee naga a agbora) produced in Uganda
(AJOL: Animal Production Research Advances., 2009) Wawa, SA; Sserunjogi, ML; Ogwok, P; Mugampoza, D
Different types of indigenous fermented milks are widely consumed in pastoralist communities of Africa, including Uganda. Local processing of milk generally takes place under uncontrolled hygienic conditions. Lee naga a agbora, a locally fermented milk product in northern Uganda, was evaluated for microbial contamination. The changes in pH, Total Plate Counts (TPC), Total Coliforms (TC), yeasts and moulds, and Escherichia coli 0157:H7, particularly its survival during different stages of fermentation were studied. The pH was found to decrease with fermentation time (6.54 to 4.54). E. coli was detected in most samples of Lee naga a agbora and its occurrence was observed throughout the fermentation period. The mean value of TPC (6.60± 0.10 log cfu ml-1) at the start of fermentation was significantly different (p<0.05) from that at 144 hours (7.91± 0.10 log cfu ml-1). The TPC was not significantly different (p>0.05) between 48 and 72 hours. The rate of decrease in TC counts in the initial fermentation period (0 to 48 hours) was lower than the later stages of fermentation (48 to 144 hours). Yeast and mould counts increased with fermentation time (6.20 to 7.42 log cfu ml-1). On the whole, there is risk associated with consumption of Lee naga a agbora given the occurrence of E. Coli.